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In Vitro - experime 0

in vitro - experiments which are carried out in a laboratory as distinct from in vivo experiments

in vivo -experiments which are carried out in live animals as distinct from in vitro experiments

immunity - the body response to a foreign antigen, either ingested as food, or as part of a foreign organism. there are two major ways the body defends itself; one is by antibody, production, the so called humeral response, as the antibodies circulate in the blood and the fluid between cells. the other is the cellular response, as it involves the cells of the immune system, the family of leucocytes. the particular leucocyte responsible for immune specificity is the lymphocyte. in total cell mass there are as many lymphocytes as there are liver or brain cells. during development there are millions of b (from the bone marrow) lymphocytes made, each with a different cell membrane ligand, specific for any one of millions of antigens. the lymphocytes are circulating all the time so that they can have the chance to meet up with a foreign antigen. as soon as an antigen has been recognised by one of these cells, and bound to the cell ligand, it stimulates the cell to reproduce millions of copies of itself. all the daughter cells are clones of the original cell. these b lymphocyte daughters, migrate to the site where the antibody is needed. instead of making an antigen for the membrane these cells make large amounts of soluble antibody. they are now recognisable as plasma cells. t lymphocytes ( having spent time in the thymus) comprises the cell mediated response to an antigen. they are of two types, killer t cells and helper t cells. most t lymphocytes are helpers and they regulate the response of the b lymphocytes. the killer t cells are however capable of recognising the foreign antigen on the surface of a cell, and then killing the entire cell. the immune response is part of a less specific defense and healing response of the body known as inflammation.

indirect pulp cap - a dressing, usually calcium hydroxide, placed against the pulpal wall of a deep cavity, in order to encourage affected dentine to remineralise. the cavity is closed with a temporary filling material and re-opened after 6 weeks to assess the state of the pulp.

induction - cell differentiation which is brought about by the influence of cytokines released by cells of another type.

infected dentine- dentine which has been damaged beyond repair by caries and is infected by large numbers of caries bacteria.

inflammation - is a whole complex of events which occur in sequence, in response to injury. tissue damaged by bacteria, chemicals, heat, trauma etc, release histamine and bradykinin and serotonin which cause an increase in capillary permeability and vasodilation. both these factors contribute to the formation of a fluid exudate in the damaged tissue, which includes fibrinogen and therefore soon clots into a firm gel. this process has the effect of walling off the bacteria or toxic substances causing the damage, or at least it slows down their spread into surrounding tissues. local macrophages, begin their phagocytic activity but their numbers are small. damaged tissues also release interleukin, messengers which are transported all the way to the bone marrow, where millions of leucocytes are stored.. these stores now release leucocytes, mostly neutrophils into the blood. the neutrophils gather at the site of damage because the endothelial cells of the local capillary walls have become sticky to leucocytes. this stickiness is specific for leucocytes and is the work of selectins expressed on the cell membrane of the endothelial cell. the leucocytes begin to catch and roll along the endothelium until they are brought to a standstill. the increased permeability of the endothelial cells allows leucocytes to wriggle out of the capillary and migrate into the damaged area. this migration is also dependent on a process know as chemotaxis, in which cytokine messages from the damaged cells attract the leucocytes to come to their aid. after several days the battle zone is filled with dead bacteria, dead tissue cells, dead neutrophils and macrophages. this dead mass of tissue is called pus. the end of the event may be the gradual resorption of pus by fresh macrophages, or the pus, now under some pressure, may force its way somewhere else. pus from the apex of a tooth may escape laterally through the alveolar bone and mucosa, where it is recognisable as a "gum boil".ten days after a foreign protein is detected for the first time, the bodies immune system has mounted a more specific defense. antibodies are produced by b lymphocytes and t lymphocytes have been alerted to the invasion.

insulin - an endochrine hormone produced in the spleen which controls the amount of sugar in the blood by a) transporting it into cells and promoting glycolysis b) converting it into glycogen for storage in the liver and muscles, and c) converting it into fats. without sufficient insulin, glucose accumulates in the blood and urine and the cells of the body are starved, a condition known as diabetes. the control of insulin production is another example of a feedback system.

insulin-like growth factor (igf) - a cytokine that influences growth hormone activity.

integrins are the cell surface receptor molecules which match up with parts of the matrix protein ligands to allow adhesion of the cell to the matrix. the attachment of cells to matrix proteins also influences the cells behaviour, by the expression of genes. the integrins are a family of proteins, found doing the same job on all animal cells. their importance in maintaining the structural integrity of cells led to the name integrins.

intercalated ducts - ducts which carry saliva between the tubules and the striated ducts.

interferon - a glycoprotein produced by cell s which mobilise the t lymphocytes to inhibit viruses and the growth of cancer cells.

interleukins - a variety of compounds(about 20) that are produced by lymphocytes, macrophages, and monocytes. they regulate the cell mediated response of the immune system. interleukin-1 is involved in the triggering of the immune response, starting acute inflammation and maintaining chronic inflammation. interleukin-2 is produced by helper t cells and induces proliferation of immune cells, both t and b. interleukin-3 promotes the differentiation and proliferation of stem cells of the leucocyte family.interleukin -6 produced by various cells including tumour cells and acts as a stimulant of plasma proteins and b and t cells. interleukin -12 is produced by a range of cells. it activates t cells and natural killer cells. it promotes the response to a range of pathogens including hiv of interleukin-2. it appears to be one of the most promising interleukins for the control of viral, bacterial and protozoal infections.